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KMID : 0603820100160030187
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Journal of Experimental & Biomedical Science
2010 Volume.16 No. 3 p.187 ~ p.192
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Molecular Identification Patterns of Clinical Isolates from Korean Patients Infected with Dermatophytes
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Kim Sang-Ha
Cho Seong-Wan
Hwang Seock-Yeon
Jeon Soo-Young
Kim Young-Kwon
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Abstract
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The aim of this study was to compare the results of morphological phenotypic examination and molecular biological method, randomly amplified polymorphic DNA (RAPD) using clinical isolates from Korean patients infected with dermatophytes. A total of 44 clinical isolates have been collected by Korean Collection Medical Fungi (KCMF) and those were subjected for the RAPD analysis. The results showed that the fungal strains that have RG1 band patterns were identified as Trichophyton rubrum by the neighbour-joining analysis. Additionally, other fungal strains that have RG3 band patterns were identified as Epidermophyton floccosum and RG5 band patterns were identified as Micorsporum gypseum. Even though molecular biological method such as RAPD are not necessary, it might be useful when some strains have similar morphological characteristics or when same species has phenotypic variations. In this study, therefore, we targeted internal transcribed spacer (ITS) regions of fungal rDNA, performed RAPD and then compared with morphological phenotypic examination.
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KEYWORD
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Dermatophytes, Randomly amplified polymorphic DNA(RAPD), ITS, Fungal rDNA
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